Received: ApAccepted: JanuPublished: January 30, 2014Ĭopyright: © 2014 Xue et al. PLoS ONE 9(1):Įditor: Aernout Luttun, Katholieke Universiteit Leuven, Belgium (2014) Indian-Ink Perfusion Based Method for Reconstructing Continuous Vascular Networks in Whole Mouse Brain. This study provided an effective method for studying the entire macro and micro vascular networks of mouse brain simultaneously.Ĭitation: Xue S, Gong H, Jiang T, Luo W, Meng Y, Liu Q, et al. Besides the observations of fine and complex vascular networks in the reconstructed slices and entire brain views, a representative continuous vascular tracking has been demonstrated in the deep thalamus. The voxel resolution is 0.35×0.4×2.0 µm 3 for the whole brain. With 17 days of work, an integral dataset for the entire cerebral vessels was acquired. Here, we have combined the improved gelatin-Indian ink vessel perfusion process with Micro-Optical Sectioning Tomography for imaging the vessel network of an entire mouse brain. Simultaneous vascular studies of arteries, veins and capillaries have not been achieved in the whole brain of mammals. Limited by the restrictions of the vascular markers and imaging methods, studies on cerebral vascular structure now mainly focus on either observation of the macro vessels in a whole brain or imaging of the micro vessels in a small region. The topology of the cerebral vasculature, which is the energy transport corridor of the brain, can be used to study cerebral circulatory pathways.
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